Research papers (since 2010) - Google scholar link

  • Laura Banaszynksi, C. David Allis, and David Shechter, 2010. “Analysis of Histones and Chromatin in Xenopus laevis Egg and Oocyte Extracts.”  Methods. 51:3-10.

  • Carola Wilczek, Raghu Chitta, Eileen Woo, Jeffrey Shabanowitz, Brian Chait, Donald Hunt, and David Shechter.  2011. “Protein arginine methyltransferase PRMT5/MEP50 methylates histones H2A and H4 and the histone chaperone Nucleoplasmin in Xenopus laevis eggs.” Journal of Biological Chemistry, 286:42221-31.

  • Meng-Chiao Ho, Carola Wilczek, Jeffrey Bonnano, Li Xing, Janina Seznec, Tsutomu Matsui, Lester G. Carter, Takashi Onikubo, P. Rajesh Kumar, Michael Brenowitz, R. Holland Cheng, Ulf Reimer, Steven Almo, David Shechter. 2013. “Structure of the Arginine Methyltransferase PRMT5-MEP50 Reveals a Mechanism for Substrate Specificity”. PLoS ONE 8(2): e57008. doi:10.1371/journal.pone.0057008

  • Wei-Lin Wang, Lissa C. Anderson, Joshua J. Nicklay, Hongshan Chen, Matthew J. Gamble, Jeffrey Shabanowitz, Donald F. Hunt, David Shechter 2014. “Phosphorylation and arginine methylation mark histone H2A prior to deposition during Xenopus laevis development”. Epigenetics and Chromatin, 7:22. doi:10.1186/1756-8935-7-22.

  • Emmanuel S. Burgos, Carola Wilczek, Takashi Onikubo, Jeffrey B. Bonanno, Janina Jansong, Ulf Reimer, and David Shechter. 2015. “Histone H2A and H4 N-Terminal Tails are Positioned by the MEP50 WD-Repeat Protein for Efficient Methylation by the PRMT5 Arginine Methyltransferase.” Journal of Biological Chemistry. PMID: 25713080 doi: 10.1074/jbc.M115.636894

  • Takashi Onikubo, Joshua J. Nicklay, Li Xing, Christopher Warren, Brandon Anson, Wei-Lin Wang, Emmanuel S. Burgos, Sophie E. Ruff, Jeffrey Shabanowitz, R. Holland Cheng, Donald F. Hunt, and David Shechter. 2015. “Developmentally Regulated Post-Translational Modification of Nucleoplasmin Controls Histone Sequestration and Deposition”. Cell Reports, Mar 11. doi:10.1016/j.celrep.2015.02.038, PMID: 25772360

  • Sun J, Zhao Y, McGreal R, Cohen-Tayar Y, Rockowitz S, Wilczek C, Ashery-Padan R, Shechter D, Zheng D, Cvekl A. “Pax6 associates with H3K4-specific histone methyltransferases Mll1, Mll2, and Set1a and regulates H3K4 methylation at promoters and enhancers.” Epigenetics and Chromatin. 2016 Sep 9;9(1):37. doi: 10.1186/s13072-016-0087-z. PMID: 27617035 PMCID: PMC5018195

  • Wei-lin Wang and David Shechter. 2016. “Chromatin assembly and transcriptional cross-talk in Xenopus laevis oocyte and egg extracts.” Int J. Dev. Biol. doi: 10.1387/ijdb.160161ds

  • Hongshan Chen, Benjamin Lorton, Varun Gupta, David Shechter. 2017. “A TGFβ-PRMT5-MEP50 axis regulates cancer cell invasion through histone H3 and H4 arginine methylation coupled transcriptional activation and repression.” Oncogene, epub Jun 2016. doi: 10.1038/onc.2016.205, PMID: 27270440 PMCID: PMC5140780

  • Emmanuel S. Burgos, Ryan O. Walters, Derek M. Huffman, and David Shechter. 2017. “A simplified characterization of S-adenosyl-L-methionine-consuming enzymes with 1-Step EZ-MTase: a universal and straightforward coupled-assay for in vitro and in vivo setting.” Chem. Sci. doi: 10.1039/C7SC02830J

  • Christopher Warren, Tsutomu Matsui, Jerome M. Karp, Takashi Onikubo, Sean Cahill, David Cowburn, Michael Brenowitz, Mark Girvin and David Shechter, “Dynamic Intramolecular Regulation of the Histone Chaperone Nucleoplasmin Controls Histone Binding and Release”. Nature Communications, 2017;8(1):2215. doi: 10.1038/s41467-017-02308-3. PMID: 29263320 PMCID: PMC5738438

  • Wei-Lin Wang, Takashi Onikubo, and David Shechter. 2018. “Chromatin Characterization in Xenopus laevis cell free egg extracts and embryos”. CSHL Protocols. doi: 10.1101/pdb.prot099879

  • Ryan O. Walters, Esperanza Arias, Antonio Diaz, Emmanuel S. Burgos, Fangxia Guan, Simoni Tiano, Kai Mao, Cara L. Green, Yungping Qiu, Hardik Shah, Donghai Wang, Adam Hudgins, Tahmineh Tabrizian, Valeria Tosti, David Shechter, Luigi Fontana, Irwin J. Kurland, Nir Barzilai, Ana Maria Cuervo, Daniel E.L. Promislow, and Derek M. Huffman. 2018. “Sarcosine is uniquely modulated by aging and dietary restriction in rodents and humans”. Cell Reports. 2018 Oct 16;25(3):663-676.e6. doi: 10.1016/j.celrep.2018.09.065.

  • Mirunalini Ravichandran, Run Lei, Qin Tang, Yilin Zhao, Joun Lee, Liyang Ma, Stephanie Chrysanthou, Benjamin M.Lorton, Ales Cvekl,  David Shechter, Deyou Zheng, Meelad M. Dawlaty. “Rinf Regulates Pluripotency Network Genes and Tet Enzymes in Embryonic Stem Cells”. Cell Reports Volume 28, Issue 8, 20 August 2019, Pages 1993-2003.e5, doi:10.1016/j.celrep.2019.07.080

  • Benjamin M. Lorton, Rajesh K. Harijan, Emmanuel S. Burgos, Jeffrey B. Bonanno, Steven C. Almo, and David Shechter. “A binary arginine methylation switch on histone H3 Arginine 2 regulates its interaction with WDR5.” Biochemistry, DOI: 10.1021/acs.biochem.0c00035 • Publication Date (Web): 24 Mar 2020

  • Christopher Warren, Jeffery B. Bonanno, Steven C. Almo, David Shechter 2020. “Structure of a Single Chain H2A/H2B Dimer”. Acta Cryst. (2020). F76, 194-198, doi: 10.1107/S2053230X20004604

Reviews and book chapters (since 2010)
  • David Shechter. 2014. “Seeing Beyond the Double Helix.” Journal of Pediatric Ophthalmology & Strabismus. Vol. 51, No. 5.
  • Nicole Stopa, Jocelyn Krebs, David Shechter. 2015. “The PRMT5 Arginine Methyltransferase: Many Roles in Development, Cancer, and Beyond.” Cellular and Molecular Life Sciences, Feb 7. (11):2041-59. PMID: 25662273 PMCID: PMC4430368
  • Takashi Onikubo and David Shechter. 2016. “Chaperone-mediated chromatin assembly and transcriptional regulation in Xenopus laevis.” Int J. Dev. Biol. doi: 10.1387/ijdb.130188ds
  • Christopher Warren and David Shechter, 2017. “Fly Fishing for Histones: Catch and Release by Histone Chaperone Intrinsically Disordered Regions and Acidic Patches”, J. Mol Biol 429(16):2401-2426. doi: 10.1016/j.jmb.2017.06.005. PMID: 28610839 PMCID: PMC5544577 *
  • Benjamin Lorton and David Shechter. 2019. “Cellular Consequences of Arginine Methylation” Cell. Mol. Life Sci. 76, 2933–2956 (2019). https://doi.org/10.1007/s00018-019-03140-2
Current preprints (prior preprints are listed in published papers)

  • Stephanie M. Lehman, Hongshan Chen, Emmanuel S. Burgos, Maxim Maron, Sitaram Gayatri, Edward Nieves, Dina L. Bai, Simone Sidoli, Varun Gupta, Matthew R. Marunde, James R. Bone, Zu-Wen Sun, Mark T. Bedford, Jeffrey Shabanowitz, Donald F. Hunt, David Shechter 2020. “Transcriptomic and proteomic regulation through abundant, dynamic, and independent arginine methylation by Type I and Type II PRMTs”. bioRxiv doi: https://doi.org/10.1101/2020.06.23.167601